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Soluble periplasmic production of human granulocyte colony-stimulating factor (G-CSF) in Pseudomonas fluorescens

✍ Scribed by Hongfan Jin; Greg T. Cantin; Steven Maki; Lawrence C. Chew; Sol M. Resnick; Jerry Ngai; Diane M. Retallack


Book ID
116829304
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
666 KB
Volume
78
Category
Article
ISSN
1046-5928

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A chemiluminescence sandwich enzyme immunoassay, using a glucose oxidase (GO) label, was developed for detecting attomole amounts o f human granulocyte colony stimulating factor (G-CSF). Purified goat F(ab')\* immobilized on a bead and purified goat Fab' labelled with GO were selected in combination