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Solubilization of membrane proteins by sulfobetaines, novel zwitterionic surfactants

✍ Scribed by Amnon Gonenne; Robert Ernst

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 709 KB
Volume: 87
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90565-1

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✦ Synopsis

A homologous series of novel zwitterionic detergents, sulfobetaines (SB,), was examined for its ability to emulsify a triglyceride model system and to extract proteins from 3T6 mouse fibroblast membranes. In both instances the solubilization efficiency of SB,s was found to improve with increasing alkyl chain length (n). The higher alkyl SB,s (n 3 12) were shown to be superior to nonionic detergents of the polyoxyethelene type (e.g., Nonidet P-40) but inferior to the anionic SDS in their ability to solubilize 3T6 cell membrane proteins. However, unlike SDS, SB,s apparently do not denature either water-soluble or membrane proteins, as judged by retention of enzymatic activity.

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