𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Solid-phase peptide synthesis using nanoparticulate amino acids in water

✍ Scribed by Keiko Hojo; Hideki Ichikawa; Mitsuko Maeda; Shinya Kida; Yoshinobu Fukumori; Koichi Kawasaki

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
207 KB
Volume
13
Category
Article
ISSN
1075-2617

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Solid‐phase peptide synthesis has many advantages compared with solution peptide synthesis. However, this procedure requires a large amount of organic solvents. Since safe organic solvent waste disposal is an important environmental problem, a technology based on coupling reaction of suspended nanoparticle reactants in water was studied. Fmoc‐amino acids are used widely, but most of them show low solubility in water. We prepared well‐dispersible Fmoc‐amino acid nanoparticles in water by pulverization using a planetary ball mill in the presence of poly(ethylene glycol). Leu‐enkephalin amide was prepared successfully using the nanoparticulate Fmoc‐amino acid on a poly(ethylene glycol)‐grafted Rink amide resin in water. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Peptide synthesis ‘in water’ by a soluti
Peptide synthesis ‘in water’ by a solution-phase method using water-dispersible nanoparticle Boc-amino acid
✍ Keiko Hojo; Hideki Ichikawa; Mare Onishi; Yoshinobu Fukumori; Koichi Kawasaki 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 299 KB

## Abstract Regulatory pressure has compelled the chemical manufacturing industry to reduce the use of organic solvents in synthetic chemistry, and there is currently a strong focus on replacing these solvents with water. Here, we describe an efficient in‐water solution‐phase peptide synthesis meth