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Background: Ischaemia-reperfusion injury (IRI) is a major cause of morbidity which may be minimized by pharmacological preconditioning. Calcium has been shown to protect cardiac muscle from IRI as well as from arrhythmia related to hyperkalaemia. Cromakalim (K + channel opener) was shown to preserve skeletal muscle microcirculation after ischaemia. The effects of both treatments on the definitive endpoint of muscle infarction were compared. Methods: Twenty-four adult male Sprague-Dawley rats (300-350 g) were divided into four groups (n = 6) (licensed Home Office approved). Tourniquet occlusion of the right thigh for 2โข5 h was followed by 3โข5 h of reperfusion. Heart rate, blood pressure, temperature and tissue oxygenation were monitored throughout. At the end of the experiment the tibialis anterior muscle was harvested to assess the size of muscle infarction. Group I (IRI): 2โข5 h ischaemia and 3โข5 h reperfusion without preceding preconditioning; group II: as for group I, preceded by three cycles of 2โข5 min of ischaemia preconditioning (IPC); group III: as for group I, preceded by the administration of calcium chloride; group IV: as for group I, preceded by K + channel opener (levocromakalim). Results: Values for mean(s.d.) muscle infarction area (MMIA) are shown in the Table .
MMIA (%)
*Significant difference.
Calcium preconditioning increases the extent of damage in ischaemic skeletal muscle and should be used with caution in reperfusion injury. K + channel opener pretreatment significantly reduced IRI and merits further study.
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## SARS abstracts 1213 index. Splenocytes were harvested from test and control mice 2 weeks following PT removal. Antitumour cytotoxicity assessed by co-culturing splenocytes with viable 3LL tumour cells. Tumour cell death was assessed using Cytoxilux fluorogenic caspase assay. P < 0โข05 using ANOV