β Scribed by Norman Hardman
No coin nor oath required. For personal study only.
Antibodies to mitosis-specific phospho-32 VANDRE, D. D., DAVIS, F. M., RAO, proteins. In Molecular Regulation of Nuclear P. N. & BORISY, G. G. (1984). Phospho-Events in Mitosis and Meiosis (ed. R. A. proteins are components of mitotic micro-Schlegel, M. A. Halleck & P. N. Rao).
antibodies to mitotic cells. Proc. Natl. Acad.
π SIMILAR VOLUMES
A replica plating method for isolating it amoebal mutants of Physarum polycephalum has been devised. Temperature-sensitive mutations occur at a frequency after nitrosoguanidine mutagenesis of 10(-3) per survivor, are stable but are not usually expressed in the plasmodia formed from these amoebae in
Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the
Microplasmodia of P. polycephalum were grown either in batch culture, in both complex and defined media to give a 3-4 fold variation in growth rate, or in a chemostate. The protein/DNA ratio of batch cultures was almost invariant, whilst the RNA/DNA ratio increased as a non-linear function of growth