Skin Tissue Engineering: Methods and Protocols

✍ Scribed by Sophie Böttcher-Haberzeth, Thomas Biedermann

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 260
• Series: Methods in Molecular Biology 1993
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.

✦ Table of Contents

Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
Isolation and Culture of Human Keratinocytes (Sergio Cortez Ghio, Gaëtan Le-Bel, Amélie Lavoie, Danielle Larouche, Lucie Germain)....Pages 3-13
Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture (Daisuke Suzuki, Filipa Pinto, Makoto Senoo)....Pages 15-31
Isolation and Culture of Epidermal Melanocytes (Muriel Cario, Alain Taieb)....Pages 33-46
Long-Term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-β Signaling Inhibitor (Filipa Pinto, Daisuke Suzuki, Makoto Senoo)....Pages 47-59
Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells (Dongrui Ma, Seng-Teik Lee, Alvin Wen Choong Chua)....Pages 61-70
Isolation and Culture of Human Dermal Fibroblasts (Marta A. Kisiel, Agnes S. Klar)....Pages 71-78
Isolation and Culture of Human Dermal Microvascular Endothelial Cells (Jennifer Bourland, Dominique Mayrand, Nathalie Tremblay, Véronique J. Moulin, Julie Fradette, François A. Auger)....Pages 79-90
Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue (Joris A. van Dongen, Martin C. Harmsen, Hieronymus P. Stevens)....Pages 91-103
Front Matter ....Pages 105-105
Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility (Lydia Costello, Nicola Fullard, Mathilde Roger, Steven Bradbury, Teresa Dicolandrea, Robert Isfort et al.)....Pages 107-122
Three-Dimensional Epidermal Model from Human Hair Follicle-Derived Keratinocytes (Takamitsu Matsuzawa, Michiyo Nakano, Ayako Oikawa, Yuumi Nakamura, Hiroyuki Matsue)....Pages 123-137
Fabrication of a Co-Culture System with Human Sweat Gland-Derived Cells and Peripheral Nerve Cells (Matthias Brandenburger, Charli Kruse)....Pages 139-148
Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose-Derived Stem Cells, and Adipocytes (Maike Keck, Alfred Gugerell, Johanna Kober)....Pages 149-157
Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering (Andrea L. Lalley, Steven T. Boyce)....Pages 159-179
Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models (Yinghong He, Cristina Has)....Pages 181-190
Front Matter ....Pages 191-191
Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts (Regine C. Tölle, Jörn Dengjel)....Pages 193-204
Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy (Gery Barmettler, Urs Ziegler)....Pages 205-215
Methods for Assessing Scaffold Vascularization In Vivo (Jiang-Hui Wang, Jinying Chen, Shyh-Ming Kuo, Geraldine M. Mitchell, Shiang Y. Lim, Guei-Sheung Liu)....Pages 217-226
Human Reconstructed Skin in a Mouse Model (Jun Mi, Shuai Chen, Lin Xu, Jie Wen, Xin Xu, Xunwei Wu)....Pages 227-237
Pig Model to Test Tissue-Engineered Skin (Christian Tapking, Daniel Popp, Ludwik K. Branski)....Pages 239-249
Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model (Thea Fleischmann, Flora Nicholls, Miriam Lipiski, Margarete Arras, Nikola Cesarovic)....Pages 251-259
Back Matter ....Pages 261-263

✦ Subjects

Life Sciences; Regenerative Medicine/Tissue Engineering; Cell Culture