Computer-Aided Tissue Engineering: Methods and Protocols

✍ Scribed by Alberto Rainer, Lorenzo Moroni

• Publisher: Springer US;Humana
• Year: 2021
• Tongue: English
• Leaves: 191
• Series: Methods in Molecular Biology 2147
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume details protocols encompassing different aspects of computer aided design and manufacturing of 3D scaffolds and biofabricated constructs for tissue engineering applications. Chapters are divided into four parts covering optimization of scaffold architectures for computer aided tissue engineering, synthetic routes to biomaterials compatible, technological platforms and manufacturing processes, and relevant applicative scenarios. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Computer-Aided Tissue Engineering: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

✦ Table of Contents

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
Biomimetic Boundary-Based Scaffold Design for Tissue Engineering Applications (Henrique A. Almeida, Paulo J. Bártolo)....Pages 3-18
Triply Periodic Minimal Surfaces (TPMS) for the Generation of Porous Architectures Using Stereolithography (Sebastien B. G. Blanquer, Dirk W. Grijpma)....Pages 19-30
3D Printing of Functionally Graded Films by Controlling Process Parameters (Alessandra Bonfanti, Loris Domenicale, Atul Bhaskar)....Pages 31-42
Front Matter ....Pages 43-43
Photocurable Biopolymers for Coaxial Bioprinting (Marco Costantini, Andrea Barbetta, Wojciech Swieszkowski, Dror Seliktar, Cesare Gargioli, Alberto Rainer)....Pages 45-54
Synthesis of an UV-Curable Divinyl-Fumarate Poly-ε-Caprolactone for Stereolithography Applications (Alfredo Ronca, Sara Ronca, Giuseppe Forte, Luigi Ambrosio)....Pages 55-62
Nanocomposite Clay-Based Bioinks for Skeletal Tissue Engineering (Gianluca Cidonio, Michael Glinka, Yang-Hee Kim, Jonathan I. Dawson, Richard O. C. Oreffo)....Pages 63-72
Front Matter ....Pages 73-73
Additive Manufacturing Using Melt Extruded Thermoplastics for Tissue Engineering (Andrea Roberto Calore, Ravi Sinha, Jules Harings, Katrien V. Bernaerts, Carlos Mota, Lorenzo Moroni)....Pages 75-99
Computer-Aided Wet-Spinning (Dario Puppi, Federica Chiellini)....Pages 101-110
Production of Scaffolds Using Melt Electrospinning Writing and Cell Seeding (Eleonore C. L. Bolle, Deanna Nicdao, Paul D. Dalton, Tim R. Dargaville)....Pages 111-124
Low-Voltage Continuous Electrospinning: A Versatile Protocol for Patterning Nano- and Micro-Scaled Fibers for Cell Interface (Zhaoying Li, Xia Li, Yan Yan Shery Huang)....Pages 125-135
Direct-Write Deposition of Thermogels (Sara Maria Giannitelli, Valeria Chiono, Pamela Mozetic)....Pages 137-142
3D Bioprinting of Complex, Cell-laden Alginate Constructs (Atabak Ghanizadeh Tabriz, Dirk-Jan Cornelissen, Wenmiao Shu)....Pages 143-148
Surface Tension-Assisted Additive Manufacturing of Tubular, Multicomponent Biomaterials (Elia A. Guzzi, Héloïse Ragelle, Mark W. Tibbitt)....Pages 149-160
Front Matter ....Pages 161-161
Bioprinting of Complex Vascularized Tissues (Wei Zhu, Claire Yu, Bingjie Sun, Shaochen Chen)....Pages 163-173
A Scaffold Free 3D Bioprinted Cartilage Model for In Vitro Toxicology (Pallab Datta, Yang Wu, Yin Yu, Kazim K. Moncal, Ibrahim T. Ozbolat)....Pages 175-183
Back Matter ....Pages 185-188

✦ Subjects

Chemistry; Biotechnology; Regenerative Medicine/Tissue Engineering