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Skin basement membrane ultrastructure after rapid-freezing and freeze-substitution fixation

✍ Scribed by P. Valkenberg; M. Leclercq-Smekens; R. Leloup

Book ID
103915850
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
308 KB
Volume
21
Category
Article
ISSN
0739-6260

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In this article, we report on the adaptation of high-pressure freezing and freeze-substitution (HPF-FS) for ultrastructural analysis of leaf tissue with special emphasis on chloroplasts. To replace the gas in the intercellular spaces, a mixture of water and methanol (MeOH) was employed. We compared