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Ultrastructure of Sea Urchin Calcified Tissues after High-Pressure Freezing and Freeze Substitution

✍ Scribed by Laurent Ameye; René Hermann; Philippe Dubois

Book ID
115634132
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
625 KB
Volume
131
Category
Article
ISSN
1047-8477

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In this article, we report on the adaptation of high-pressure freezing and freeze-substitution (HPF-FS) for ultrastructural analysis of leaf tissue with special emphasis on chloroplasts. To replace the gas in the intercellular spaces, a mixture of water and methanol (MeOH) was employed. We compared