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Size separation and size determination of liposomes

✍ Scribed by Kumiko Sakai-Kato; Shigenori Ota; Kenji Hyodo; Hiroshi Ishihara; Hiroshi Kikuchi; Toru Kawanishi

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
203 KB
Volume
34
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

We developed a method for separating liposomes by size and determining their average diameters. Liposomes with different average diameters were separated on a monolithic silica capillary column, and the size of the liposomes corresponding to each peak was determined online with a dynamic light scattering detector coupled to the capillary liquid chromatography system. The calculated diameters for the separated liposomes were similar to the diameter values measured in batch mode. We demonstrate that this combination of a monolithic capillary column and light scattering detection could be used for size separation of liposomes and could provide more details about average diameters than batch‐mode analysis.

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