Size fractionation of double-stranded DNA by precipitation with polyethylene glycol
β Scribed by Lis, John T. (author);Schleif, Robert (author)
- Book ID
- 121679437
- Publisher
- Oxford University Press
- Year
- 1975
- Tongue
- English
- Weight
- 766 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0305-1048
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Recently, a new radioimmunoassay-the polyethylene glycol (PEG) assay-was introduced to measure antibodies to double-stranded (ds) DNA. In this method, polyethylene glycol precipitation of formed 3H-DNMantiDNA complexes is used instead of the ammonium sulfate precipitation used in the Farr assay. In
The precipitation of proteins with polyethylene glycol (PEG) of various average molecular weights was studied. it was found that the specificity of precipitation, that is the ratio of desired protein and total protein, can be improved by using PEG fractions of lower average molecular weight than the