Taken Found Recovery, Yoa 1.96 1.95 99.5 2.91 2.98 102.4 3.85 3.81 99.0 4.76 4.63 97.3 a Mean f standard deviation = 0.652 f 0.027. Absorbance at 570 mp follows Beer's law over the range 0.10 to 0.50 mg. of chlorphenesin per sample (Table 11). A mean absorbance per milligram i standard deviation of
Selectivity of protein precipitation with polyethylene glycol fractions of various molecular weights
✍ Scribed by Werner Hönig; Maria-Regina Kula
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 616 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The precipitation of proteins with polyethylene glycol (PEG) of various average molecular weights was studied. it was found that the specificity of precipitation, that is the ratio of desired protein and total protein, can be improved by using PEG fractions of lower average molecular weight than the generally employed PEG 6000. Specificity increases with decreasing average molecular weight of PEG. Purification of oc-glucosidase from crude yeast extract by fractional precipitation with PEG 400 is found comparable to gel chromatography.
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