Size-exclusion reaction chromatography (SERC): A new technique for protein PEGylation
โ Scribed by Conan J. Fee
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 160 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0006-3592
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๐ SIMILAR VOLUMES
A high-performance size-exclusion chromatographic system is described that minimizes the ionic and hydrophobic interactions of proteins with the stationary phase. The system can be used to determine reliably the molecular weight of proteins between M, 10,000 and 70,000. A further application would b
HPLC was used in combination with immuno-bead separation technique for identification of an individual protein from a pool of proteins. This was carried out using an in-house monoclonal antibody (ATC2) specific for placental alkaline phosphatase (PLAP) as a primary antibody for conjugation to CNBr b