In our hands, we occasionally observed negative-going reconstructed size-exclusion chromatograms in SEC/ESI-MS which are possibly related to polymer type, salt concentration and/or other operating conditions. In the IcniM?.
Size exclusion chromatography – A blessing and a curse of science and technology of synthetic polymers
✍ Scribed by Dušan Berek
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 339 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1615-9306
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Size exclusion chromatography, SEC is one of the most popular methods for the separation of different kinds of macromolecules. This critical review gives concise information about macromolecules and their behavior in solution, basic understanding about principles, instrumentation, and application possibilities of SEC, and more in detail discusses drawbacks and pitfalls of the method with the emphasis on synthetic polymers. Selected practical advices are included to help enhance the quality of SEC results.
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