Two site-specific mutations of dihydrofolate reductase from Escherichiu coli based on the x-ray crystallographic structure were constructed. The first mutation (His-45 + Gln) is aimed at assessing the interaction between the imidazole moiety and the pyrophosphate backbone of NADPH. The second (Thr-1
Site-specific mutagenesis of large DNA viral genomes
โ Scribed by Frank J. Jenkins; Dr Bernard Roizman
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 488 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0265-9247
No coin nor oath required. For personal study only.
โฆ Synopsis
Site-specijic or target-specijic mutagenesis of viral DNA genomes, using a selectable marker system i s a powerful tool for the analysis of the function of specific regions of large DNA genomes. Through these techniques the construction of vectors capable of a'elivering vaccines for the prevention of infectious disease in humans and animals is possible.
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