๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Site-specific mutagenesis of large DNA viral genomes

โœ Scribed by Frank J. Jenkins; Dr Bernard Roizman


Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
488 KB
Volume
5
Category
Article
ISSN
0265-9247

No coin nor oath required. For personal study only.

โœฆ Synopsis


Site-specijic or target-specijic mutagenesis of viral DNA genomes, using a selectable marker system i s a powerful tool for the analysis of the function of specific regions of large DNA genomes. Through these techniques the construction of vectors capable of a'elivering vaccines for the prevention of infectious disease in humans and animals is possible.


๐Ÿ“œ SIMILAR VOLUMES


Site-specific mutagenesis of dihydrofola
โœ Jin-Tann Chen; Ruth J. Mayer; Carol A. Fierke; Stephen J. Benkovic ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 441 KB

Two site-specific mutations of dihydrofolate reductase from Escherichiu coli based on the x-ray crystallographic structure were constructed. The first mutation (His-45 + Gln) is aimed at assessing the interaction between the imidazole moiety and the pyrophosphate backbone of NADPH. The second (Thr-1

Totalin VitroSynthesis of Plasmid DNA Fa
โœ Rolf Postina; Falk Fahrenholz ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 89 KB

and automodified (5) during activity blotting. Consis-8. Ikai, K., Ueda, K., and Hayaishi, O. (1982) in ADP-Ribosylation tent with this result, we observed that C-2-10 readily Reactions: Biology and Medicine (Hayaishi, O., and Ueda, K., labeled the blots after activity-Western blot and era-Eds.), pp

Site-specific mutagenesis of cDNA clones
โœ Bert L. Semler; Victoria H. Johnson; Patricia Gillis Dewalt; Mary Frances Ypma-W ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 829 KB

The cleavage of poliovirus precursor polypeptides occurs at specific amino acid pairs that are recognized by viral proteinases. Most of the polio-specific cleavages occur at glutamine-glycine (Q-G) pairs that are recognized by the viral-encoded proteinase 3C (formerly called P3-7c). In order to carr

Topological aspects of site-specific DNA
โœ Roland Kanaar; Pieter van de Putte ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 817 KB

Sum ma ry ## Site-spec@ recombination events are of fundamental importance in many biological systems. In vitro experiments using purified proteins and D N A substrates are yielding insights into strand exchange mechanisms and synapsis of recombination sites. By examining results across a range o