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Totalin VitroSynthesis of Plasmid DNA Facilitates Site-Directed Mutagenesis

โœ Scribed by Rolf Postina; Falk Fahrenholz


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
89 KB
Volume
232
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


and automodified (5) during activity blotting. Consis-8. Ikai, K., Ueda, K., and Hayaishi, O. (1982) in ADP-Ribosylation tent with this result, we observed that C-2-10 readily Reactions: Biology and Medicine (Hayaishi, O., and Ueda, K., labeled the blots after activity-Western blot and era-Eds.), pp. 339-360, Academic Press, New York.


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In vitro mutagenesis of large genes has proven to be difficult for a number of reasons, including the number of steps involved and the instability of large inserts. We describe here a single-step PCR method to introduce mutations into an ataxia-telangiectasia mutated (ATM) gene cDNA construct (20 kb