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Site-specific incorporation of 5-methylaminomethyl-2-thiouridine and 2-thiouridine(s) into RNA sequences

✍ Scribed by Grazyna Leszczynska; Jakub Pięta; Piotr Leonczak; Agnieszka Tomaszewska; Andrzej Malkiewicz

Book ID
113930428
Publisher
Elsevier Science
Year
2012
Tongue
French
Weight
262 KB
Volume
53
Category
Article
ISSN
0040-4039

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## Abstract The synthesis of 5‐methylaminomethyl‐2‐thiouridine **(1)**, a rare nucleoside from t‐RNA, starting from the appropriate 2‐thiouracil and ribose derivatives is described. During the silyl Hilbert‐Johnson nucleoside synthesis with silver perchlorate the __tert__‐butoxycarbonyl (Boc) amino

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The structure of the title compound 1 has been determined by X-ray analysis. The following crystal data were found: orthorhombic, P2J2J.?1, a = 5.243(2), b = 24.864(4), and c = 37.083(6) A. Three molecules of the compound with four molecules of water are present in the independent part of the unit c