𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Site-Selective Affinity Labelling of Maltose Binding Protein in Bacterial Cells

✍ Scribed by Dr. Anthony Rullo; Andrew A. Beharry; Rodolfo F. Gómez-Biagi; Xiong Zhao; Prof. Mark Nitz


Book ID
112016146
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
713 KB
Volume
13
Category
Article
ISSN
1439-4227

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Selection of High-Affinity Binding Sites
✍ S. Pierrou; S. Enerback; P. Carlsson 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 695 KB

We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble