Single-tube reverse transcription and heminested polymerase chain reaction of hepatitis C virus RNA to detect viremia in serologically negative hemodialysis patients
β Scribed by M. Sampietro; S. Salvadori; N. Corbetta; S. Badalamenti; G. Graziani; G. Fiorelli
- Book ID
- 110573438
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 464 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0940-5437
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The combination of reverse transcription and polymerase chain reaction is a very powerful tool for the detection of hepatitis C virus RNA in sera of patients with hepatitis C virus infection. However, when studying the presence of this virus in tissue using polymerase chain reaction, it may be diffi
## Abstract A procedure is described for the detection of hepatitis C virus (HCV) RNA in blood by means of the polymerase chain reaction (PCR) in which the reverse transcription step and two rounds of amplification are carried out in a single tube. This results in fewer manipulations, reduced risk