Comparison of transcription mediated amplification (TMA) and reverse transcription polymerase chain reaction (RT-PCR) for detection of hepatitis C virus RNA in liver tissue
✍ Scribed by Wolf Peter Hofmann; Volker Dries; Eva Herrmann; Barbara Gärtner; Stefan Zeuzem; Christoph Sarrazin
- Book ID
- 116630699
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 161 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1386-6532
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Three PCR methods based on the GB virus-C/ hepatitis G virus (GBV-C/HGV) 5ЈUTR and NS3 genomic region were used for the detection of GBV-C/HGV RNA in serum of 62 patients with chronic hepatitis C virus (HCV) infection. Ten of 62 (16%) patients were found to have GBV-C/ HGV RNA, which was confirmed b
The combination of reverse transcription and polymerase chain reaction is a very powerful tool for the detection of hepatitis C virus RNA in sera of patients with hepatitis C virus infection. However, when studying the presence of this virus in tissue using polymerase chain reaction, it may be diffi