Single-tube and nested reverse transcriptase–polymerase chain reaction for detection of Rift Valley fever virus in human and animal sera

Comparison of real-time reverse transcri

Comparison of real-time reverse transcriptase–polymerase chain reaction and nested or commercial reverse transcriptase–polymerase chain reaction for the detection of hepatitis E virus particle in human serum

✍ Jeong-min Ahn; Nabin Rayamajhi; Sang Gyun Kang; Han Sang Yoo 📂 Article 📅 2006 🏛 Elsevier Science 🌐 English ⚖ 188 KB

Comparison of real-time reverse transcri

Comparison of real-time reverse transcriptase–polymerase chain reaction and nested or commercial reverse transcriptase–polymerase chain reaction for the detection of hepatitis E virus particle in human serum [Diagnostic Microbiology and Infectious Disease 56, 269–274, 2006]

✍ Jeong-min Ahn; Nabin Rayamajhi; Sang Gyun Kang; Han Sang Yoo 📂 Article 📅 2007 🏛 Elsevier Science 🌐 English ⚖ 77 KB

Quantitation of porcine reproductive and

Quantitation of porcine reproductive and respiratory syndrome virus RNA in semen by single-tube reverse transcription-nested polymerase chain reaction

✍ Jinho Shin; Elida M Bautista; Yung-Bai Kang; Thomas W Molitor 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 423 KB

Production of monoclonal antibodies agai

Production of monoclonal antibodies against Rift Valley fever virus: Application for rapid diagnosis tests (virus detection and ELISA) in human sera

✍ A. Zaki; D. Coudrier; A.I. Yousef; M. Fakeeh; M. Bouloy; A. Billecocq 📂 Article 📅 2006 🏛 Elsevier Science 🌐 English ⚖ 252 KB

Nested polymerase chain reaction for det

Nested polymerase chain reaction for detection of human immunodeficiency virus type 1 DNA in clinical specimens

✍ Maurizio Zazzi; Laura Romano; Agostina Brasini; Pier Egisto Valensin 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 345 KB

## Abstract A highly sensitive two‐step polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIV‐1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna

Detection of human metapneumovirus in re

Detection of human metapneumovirus in respiratory secretions by reverse-transcriptase polymerase chain reaction, indirect immunofluorescence, and virus isolation in human bronchial epithelial cells

✍ R.E. Ingram; F. Fenwick; R. McGuckin; A. Tefari; C. Taylor; G.L. Toms 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 136 KB

## Abstract Over two winters in Newcastle upon Tyne, respiratory secretions, negative by immunofluorescence staining for other respiratory viruses, were tested for the presence of human metapneumovirus (HMPV) by RT‐PCR. In the second winter, specimens were also tested by immunofluorescence staining