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Quantitation of porcine reproductive and respiratory syndrome virus RNA in semen by single-tube reverse transcription-nested polymerase chain reaction

✍ Scribed by Jinho Shin; Elida M Bautista; Yung-Bai Kang; Thomas W Molitor


Book ID
114140587
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
423 KB
Volume
72
Category
Article
ISSN
0166-0934

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## Abstract A procedure is described for the detection of hepatitis C virus (HCV) RNA in blood by means of the polymerase chain reaction (PCR) in which the reverse transcription step and two rounds of amplification are carried out in a single tube. This results in fewer manipulations, reduced risk