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Single-step purification of urease by affinity chromatography

โœ Scribed by Wong, Bassanio L.; Shobe, Charles R.

Book ID
120605012
Publisher
NRC Research Press
Year
1974
Tongue
French
Weight
771 KB
Volume
20
Category
Article
ISSN
0008-4166

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Rat pancreatic and salivary amylase [( 1 -4) a-D-gkan glucanohydrolase] (EC 3.2.1.1) were purified 20-to 50-fold with a yield above 75% by a single-step affinity chromatographic procedure. The affinity ligand was an a-glucohydrolase inhibitor (proprietary name Bay g5421) coupled to w-aminohexyl-Seph