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Calf lens phosphofructokinase: Purification by single-step affinity chromatography and partial characterization

โœ Scribed by John K. Wolfe; Benigno D. Peczon; Leo T. Chylack Jr


Book ID
117100497
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
808 KB
Volume
32
Category
Article
ISSN
0014-4835

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Rat pancreatic and salivary amylase [( 1 -4) a-D-gkan glucanohydrolase] (EC 3.2.1.1) were purified 20-to 50-fold with a yield above 75% by a single-step affinity chromatographic procedure. The affinity ligand was an a-glucohydrolase inhibitor (proprietary name Bay g5421) coupled to w-aminohexyl-Seph