A single-step purification of rat pancre
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Peter H. Burrill; Patsy M. Brannon; Norman Kretchmer
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Article
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1981
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Elsevier Science
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English
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Rat pancreatic and salivary amylase [( 1 -4) a-D-gkan glucanohydrolase] (EC 3.2.1.1) were purified 20-to 50-fold with a yield above 75% by a single-step affinity chromatographic procedure. The affinity ligand was an a-glucohydrolase inhibitor (proprietary name Bay g5421) coupled to w-aminohexyl-Seph