We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only
Single-Site Polymerase Chain Reaction through Single Oligonucleotide Ligation
β Scribed by Zhenwu Lin; Yunsong Zhu; Xiangfeng Cui; Honghua Li
- Book ID
- 102558851
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 92 KB
- Volume
- 231
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
DNA microarray technology holds significant promise for human DNA diagnostics. A number of technical approaches directed at the parallel identification of mutations or single nucleotide polymorphisms make use of polymerase-based specificity, like minisequencing or allele-specific primer elongation.
This study introduces a DNA microarray-based genotyping system for accessing single nucleotide polymorphisms (SNPs) directly from a genomic DNA sample. The described one-step approach combines multiplex amplification and allele-specific solid-phase PCR into an on-chip reaction platform. The multiple