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Polymerase chain reaction (PCR) amplification with a single specific primer

✍ Scribed by Miklos Kalman; Eva T. Kalman; Michael Cashel


Book ID
115763637
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
309 KB
Volume
167
Category
Article
ISSN
0006-291X

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We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only