Single-nucleotide resolution of RNAs up to 59 nucleotides by high-performance liquid chromatography
โ Scribed by Huang, Zhen; Jayaseelan, Sabarinath; Hebert, Jeffrey; Seo, Hyojung; Niu, Li
- Book ID
- 120340627
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 859 KB
- Volume
- 435
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Chromatography of AMP, NAD+, or NADH on a reverse-phase C,, Porasil B column rapidly removes ammonium formate or potassium phosphate from 90% of the nucleotide. Earlier reports showed these salts could not be separated from nucleotides by conventional desalting using gel filtration.
Procedures for the complete extraction and isocratic high-performance liquid chromatography analysis of cellular nucleoside di-and triphosphates are reported. Maximum nucleotide recovery from cultures of the ciliate Tetrahymenapyriformis is achieved if (a) cells are cooled to 4ยฐC prior to harvest; (