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Single Nucleotide Polymorphisms: Methods and Protocols (Methods in Molecular Biology, Vol. 212)

โœ Scribed by Pui-Yan Kwok

Publisher
Humana Press
Year
2002
Tongue
English
Leaves
276
Category
Library
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โœฆ Synopsis

Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

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