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Single nucleotide polymorphism genotyping: biochemistry, protocol, cost and throughput

✍ Scribed by Chen, X; Sullivan, P F

Book ID
110075248
Publisher
Nature Publishing Group
Year
2003
Tongue
English
Weight
346 KB
Volume
3
Category
Article
ISSN
1470-269X

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The large number of single nucleotide polymorphism (SNP) markers available in the public databases makes studies of association and fine mapping of disease loci very practical. To provide information for researchers who do not follow SNP genotyping technologies but need to use them for their researc

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Background: Genetic diversity can help explain disease susceptibility and differential drug response. The most common type of variant is the single nucleotide polymorphism (SNP). We present a low-cost, high throughput assay for SNP genotyping. Methods: The assay uses oligonucleotide probes covalentl