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Single-molecule analysis of DNA replication in Xenopus egg extracts

โœ Scribed by Hasan Yardimci; Anna B. Loveland; Antoine M. van Oijen; Johannes C. Walter

Book ID
118507055
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
665 KB
Volume
57
Category
Article
ISSN
1046-2023

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โœ Fenfei Leng; Gregory H. Leno ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 308 KB ๐Ÿ‘ 2 views

We have used Xenopus egg extracts to investigate the effects of the antitumor drug daunomycin on DNA replication in vitro. Xenopus sperm nuclei replicated nearly synchronously in our egg extracts, thereby allowing us to determine the effects of the drug on both replication initiation and elongation.