Inhibition of Initiation of DNA Replication in Xenopus Egg Extracts by a Phosphatase Inhibitor, Calyculin A
โ Scribed by A. Someya; N. Tanaka; A. Okuyama
- Book ID
- 115573594
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 257 KB
- Volume
- 196
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
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We have used Xenopus egg extracts to investigate the effects of the antitumor drug daunomycin on DNA replication in vitro. Xenopus sperm nuclei replicated nearly synchronously in our egg extracts, thereby allowing us to determine the effects of the drug on both replication initiation and elongation.
Xenopus egg extracts initiate replication at specific origin sites within mammalian G1-phase nuclei. Similarly, S-phase extracts from Saccharomyces cerevisiae initiate DNA replication within yeast nuclei at specific yeast origin sequences. Here we show that Xenopus egg extracts can initiate DNA repl