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Simultaneous release and purification of gene product in an aqueous two-phase system

โœ Scribed by Osamu Ariga; Isamu Miyakawa; Taro Aota; Yoshiki Sano

Book ID
113397340
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
392 KB
Volume
77
Category
Article
ISSN
0922-338X

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๐Ÿ“œ SIMILAR VOLUMES

Simultaneous production and partition of
Simultaneous production and partition of chitinase during growth ofSerratia marcescensin an aqueous two-phase system
โœ Jyh-Ping Chen; Maw-Shyan Lee ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 337 KB

Partition and production of the extracellular chitinase from Serratia marcescens were studied in PEG/dextran aqueous two-phase systems. The enzyme partitions into the bottom phase and the cells segregate into the top phase. The best system is 2 % (w/v) PEG 20000 and 5 % (w/v) dextran T500. The cell