Simultaneous production and partition of chitinase during growth ofSerratia marcescensin an aqueous two-phase system

✍ Scribed by Jyh-Ping Chen; Maw-Shyan Lee

• Book ID: 104644928
• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 337 KB
• Volume: 8
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00152884

✦ Synopsis

Partition and production of the extracellular chitinase from Serratia marcescens were studied in PEG/dextran aqueous two-phase systems. The enzyme partitions into the bottom phase and the cells segregate into the top phase. The best system is 2 % (w/v) PEG 20000 and 5 % (w/v) dextran T500. The cell growth and enzyme production kinetics are similar in the aqueous two-phase system and in the polymerfree reference system. However, the maximum enzyme concentration in the former system is 1.5 times that in the latter one.