Simultaneous Preparation and Quantitation of Proteoglycans by Precipitation with Alcian Blue
โ Scribed by S. Bjornsson
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 825 KB
- Volume
- 210
- Category
- Article
- ISSN
- 0003-2697
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Alcian blue and toluidine blue dyes form complexes with anionic glycoconjugates (AG) such as proteoglycans (PG) and glycosaminoglycans (GAG). However, the Alcian blue-AG complexes do not readily dissociate, while the toluidine blue-AG complexes do so in salt solutions. This differential dissociation
Fluorescence from poly-P-hydroxybutyrate (PHB) inclusions inside Azotobacter vinelandii UWD cells stained with Nile blue A was shown to be proportional to PHB concentration. The intensity of the fluorescence was greatest in native, fluid inclusions and was the least in extracted, crystallized granul