Simultaneous Phenotyping of Genetic Markers for Paternity Testing

✍ Scribed by Tamaki, Y. ;Nishimukai, H. ;Kishida, T. ;Fukuda, M.

• Book ID: 104660686
• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 567 KB
• Volume: 99
• Category: Article
• ISSN: 1437-1596
• DOI: 10.1007/bf00200633

✦ Synopsis

Time-and cost-saving methods for paternity testing are described. Seventeen genetic systems were divided into six groups: (1) transferrin (Tf), factor B (Bf), and phosphoglucomutase 1 (PGM1); (2) group-specific component (Gc) or al-antitrypsin (PI) and a2HS-glycoprotein (HSGA); (3) complement components C6 and C7, factor 13B (F13B), and plasminogen (PLG); (4) haptoglobin (Hp), C8 a-7 chain (C81), and factor I (IF); (5) red cell acid phosphatase (ACP), esterase D (ESD), and glutamic-pyruvic transaminase (GPT); and (6) 6-phosphogluconate dehydrogenase (PGD) and glyoxalase I (GLO). Each group of systems was typed simultaneously by electrophoresis or isoelectric focusing (IEF) followed by staining or immunoblotting. These methods are very practical because they afford a considerable saving of time, work and expense, and facilitate semipermanent preservation of electrophoretic patterns.