Simultaneous in Vitro Protein Synthesis Using Solid-Phase DNA Template
β Scribed by Mary Kate W. DiTursi; Junhoe Cha; Matthew R. Newman; Jonathan S. Dordick
- Book ID
- 109387110
- Publisher
- American Institute of Chemical Engineers
- Year
- 2004
- Tongue
- English
- Weight
- 338 KB
- Volume
- 20
- Category
- Article
- ISSN
- 8756-7938
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π SIMILAR VOLUMES
Partially purified cell-free extracts prepared from growing cells of the yeast Saccharomyces cerevisiae catalyze DNA synthesis directed by the chimeric plasmid BTYP-2 containing the whole 2 ΞΌm DNA sequence. The biochemical properties of the reaction have been examined and inactivation studies indica
Transcription factor one (TF1), a protein synthesized after infection of B. subtilis by phage SPO1, is a specific inhibitor of SPO1 in vitro transcription. In this paper, we investigate the effect of TF1 on SPO1-specific in vitro protein synthesis, using SPO1 DNA or messenger RNA as templates. Prote