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Bacteriophage SPO1 DNA- and RNA-directed protein synthesis in vitro: The effect of TF1, a template-selective transcription inhibitor

✍ Scribed by Shub, David A. ;Johnson, Gerald G.

Publisher: Springer
Year: 1975
Tongue: English
Weight: 510 KB
Volume: 137
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00341682

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✦ Synopsis

Transcription factor one (TF1), a protein synthesized after infection of B. subtilis by phage SPO1, is a specific inhibitor of SPO1 in vitro transcription. In this paper, we investigate the effect of TF1 on SPO1-specific in vitro protein synthesis, using SPO1 DNA or messenger RNA as templates. Protein syhthesis is measured by incorporation of radioactive amino acids into acid insoluble form, synthesis of a phage-specific enzymatic activity, and analysis of radioactive polypeptides by acrylamide gel electrophoresis. TF1 is shown to inhibit the DNA-dependent synthesis of all SPO1 proteins which can be made in vitro, but to have no specific effect on RNA-dependent protein synthesis in vitro.

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