Simultaneous hydrolysis of tri- and tetrasaccharides by industrial mixtures of glucoamylase and α-amylase: kinetics and thermodynamics

✍ Scribed by A. Converti; G. Fiorito; M. Borghi; G. Ferraiolo

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 595 KB
• Volume: 7
• Category: Article
• ISSN: 1615-7605
• DOI: 10.1007/bf00387412

✦ Synopsis

The present paper deals with the study of the kinetics and thermodynamics of batch enzymic hydrolysis of multisubstrate media in the presence of more than one glucanase. A kinetic model, similar to the one proposed for competitive inhibition, is presented to describe the competition between two substrates, tri-and tetrasaccharides, for the same enzyme, glucoamylase. A literature research on the most recurrent values of the Michaelis-Menten constant also shows a linear relationship between this parameter and the molecular weight of the sugar substrate for a given glucanase.