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Simultaneous genotyping for all three known structural mutations in the human mannose-binding lectin gene

โœ Scribed by D. Jack; J. Bidwell; M. Turner; N. Wood


Book ID
114248058
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
178 KB
Volume
49
Category
Article
ISSN
0378-3782

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Simultaneous genotyping for all three kn
โœ Dominic Jack; Jeffrey Bidwell; Malcolm Turner; Nigel Wood ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 194 KB

We describe a rapid and simple method for genotyping the three known structural mutations within exon 1 of the mannan-binding lectin (MBL) gene. A PCR-amplifiable synthetic DNA (Universal Heteroduplex Generator) was annealed to genomic PCR product from exon 1 to generate unique DNA heteroduplexes fo