Simultaneous genotyping for all three known structural mutations in the human mannose-binding lectin gene
✍ Scribed by Dominic Jack; Jeffrey Bidwell; Malcolm Turner; Nigel Wood
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 194 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1059-7794
No coin nor oath required. For personal study only.
✦ Synopsis
We describe a rapid and simple method for genotyping the three known structural mutations within exon 1 of the mannan-binding lectin (MBL) gene. A PCR-amplifiable synthetic DNA (Universal Heteroduplex Generator) was annealed to genomic PCR product from exon 1 to generate unique DNA heteroduplexes for each mutation. Heteroduplexes were then resolved by non-denaturing polyacrylamide gel electrophoresis. The technique was initially validated with previously typed samples and then applied to previously untyped samples with the results confirmed by DNA sequencing. Hum Mutat 9:41-46, 1997.