Simultaneous gas chromatographic determination of the cumene metabolites 2-phenylpropanol-1 and 2-phenylpropanol-2 in urine

✍ Scribed by S. Goenechea; K. Olek; P. Wardenbach

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 185 KB
• Volume: 154
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98479-6

✦ Synopsis

Cumene is a common constituent of many petroleum distillates. It is widely used as a thinner for paints and enamels and has become a commercial source of phenol and acetone. The acute toxicity of cumene is greater than that of benzene and toluene: rats exposed to cumene developed hyperemia and congestion in the lungs, liver and kidneys'. Experiments on rabbits showed that an oral dose of cumene is metabolized in the liver: 40 % is converted into Z-phenyfpropanol-2 (2-P-P-2), 25 % into 2-phenylpropanoi-1 (2-P-P-l) and 25 o/0 into 2-phenylpropionic acid. These metabolites are excreted in the urine as glucuronides'.

After exposure to cumene vapour for 8 h, man converts 35 % of the absorbed cumene into 2-P-P-2 within 48 h. The urinary metabolite was determined by gas chromatography3.

This paper describes the simultaneous gas chromatographic determination of 2-P-P-2 and 2-P-P-1 in urine. EXPERIMENTAL