Simultaneous gas chromatographic analysis for the four commonly used antiepileptic drugs in serum

✍ Scribed by Chempithera Varughese Abraham; Harry Douglas Joslin

• Book ID: 104145554
• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 419 KB
• Volume: 128
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)99259-8

✦ Synopsis

We describe a simple, sensitive method for the determination of phenobarbital, diphenylhydantoin, carbamazepine and primidone in serum, by use of gasliquid chromatography with temperature programming. The methylated derivatives of these anticonvulsants were well resolved, as was 5(p-methylphenyl)-5-phenylhydantoin, the internal standard. In this procedure we used an ion-exchange resin for separation of the drug from the serum. The proposed procedure requires only 1.0 ml of serum and can be done in Iess than 1 h. The lower limit of detection for each of rhe drugs is 0.5 mg/I. Analytical recoveries of drug from serum were excellent and peak height and concentration were linearly related up to twice the toxic concentration for serum.