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Simultaneous expression of genes encoding endoglucanase and β-glucosidase inZymomonas mobilis

✍ Scribed by P. Su; C. -Q Liu; R. J. Lucas; S. F. Delaney; N. W. Dunn

Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
676 KB
Volume
15
Category
Article
ISSN
0141-5492

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✦ Synopsis

As a step towards constructing strains of Z. mobilis capable of converting cellulose to ethanol, DNA fragments encoding endoglucanase (from Xunthomonus albilineans) and /3-glucosidase (from either X.aZbiZineuns or Pseudomonus sp.) were linked on the same vector and transferred to Z. mobilis. All clones expressed endoglucanase. p-Glucosidase was only produced by clones containing the Xunthomonus gene, and when two copies of this gene were present the pglucosidase activity was higher.

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✍ Jeong Hyun Kim; Baek-Rak Lee; M. Y. Pack 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 291 KB

Simultaneous overproduction of intracellular B-glucosidase and extracellular endoglucanase was attempted by constructing two artificial operon systems comprising the B-glucosidase-endoglueanase gene(BE) or the endoglucanase-B-glucosidase gene(EB) under the control of a strong engineered promoter, BJ