Simultaneous overproduction of extracellular endoglucanase and intracellular β-glucosidase by genetically engineeredBacillus subtilis

✍ Scribed by Jeong Hyun Kim; Baek-Rak Lee; M. Y. Pack

• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 291 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00143113

✦ Synopsis

Simultaneous overproduction of intracellular B-glucosidase and extracellular endoglucanase was attempted by constructing two artificial operon systems comprising the B-glucosidase-endoglueanase gene(BE) or the endoglucanase-B-glucosidase gene(EB) under the control of a strong engineered promoter, BJ27UA88 and expressing them in Bacillus subtilis DB104. Two artificial operon systems contained 30 bp or 5 bp gap between the termination codon of the upstream gene and the SD sequence of the downstream gene, respectively. These operon systems were expressed well in B. subtilis and overproduced the B-glucosidase cell extract as well as the endoglucanase supematant. The level of expression in the operon system was almost the same as that in a single expression system.