𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Simultaneous evaluation for terminal deoxynucleotidyl transferase and myeloperoxidase in leukemia

✍ Scribed by Dr. J. D. Folds; F. J. Bollum; L. Dean; J. Chan; S. A. Stass

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
791 KB
Volume
12
Category
Article
ISSN
0361-8609

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A technique for dual staining of cells using terminal deoxynucleotidyl transferase (TdT) and myeloperoxidase (MPO) is described. The technique has been applied to cells of two patients. One patient had chronic myelomonocytic leukemia evolving into acute myelomonocytic leukemia. The other patient had chronic myelogenous leukemia in blast crisis. Our findings indicate that TdT and MPO are exclusive markers except for a rare precursor cell with dual staining in one patient. This study supports the concept of acute mixed leukemia.

πŸ“œ SIMILAR VOLUMES

High terminal deoxynucleotidyl transfera
High terminal deoxynucleotidyl transferase activity in pediatric patients with acute lymphocytic and acute myelocytic leukemias
✍ B. I. Sahai Srivastava; Sohaib A. Khan; Jun Minowada; Arnold Freeman πŸ“‚ Article πŸ“… 1978 πŸ› John Wiley and Sons 🌐 French βš– 517 KB

## Abstract Specificity of TdT^5^ as a marker for ALL was evaluated by determining its activity in cells from normal control subjects and from 35 pediatric patients with ALL, AML, Hodgkin's disease and disseminated Burkitt's lymphoma. We evaluated the DNA polymerase activity, cell surface pheno‐typ

An unusual artefact in the terminal deox
An unusual artefact in the terminal deoxynucleotidyl transferase assay for apoptotic cells
✍ S. Detre; M. G. Ormerod; J. C. Titley; M. Dowsett πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 89 KB πŸ‘ 1 views

A staining artefact associated with the terminal deoxynucleotidyl assay for apoptotic cells is described. The artefact is caused by particulate matter in the reconstituted dried milk used in the wash-ing buffer. We recommend filtering the washing buffer before use.