Simultaneous determination of vitamins A and E in feeds and foods by reversed phase high-pressure liquid chromatography

✍ Scribed by Petter Söderhjelm; Bernt Andersson

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 285 KB
• Volume: 29
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/jsfa.2740290807

✦ Synopsis

Abstract

A simple high‐pressure liquid chromatographic method for the simultaneous determination of vitamins A and E in animal feeds and foods has been developed and evaluated. After saponification and extraction the sample was run on a reversed‐phase column with water‐methanol as the mobile phase. The detector was an u.v.‐lamp at 280 nm. The method has been tested on different types of feeds and foods containing between 2‐30 μg vitamin A g^−1^ of sample and 5‐300 μg vitamin E g^−1^ of sample. The recovery of added vitamins was 86 % for retinylacetate and 91 % for α‐tocopheryl‐acetate with a standard deviation of 3 % for both. Results of the proposed procedure agreed well with those obtained by other methods.