Simultaneous Determination of Ultratrace Prostaglandins in Biological Samples with High-Performance Liquid-Chromatography/Laser-Induced Fluorometry

✍ Scribed by S. Sakae; A. Harata; T. Kitamori; T. Sawada; A. Okubo; S. Toda; T. Shimizu

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 320 KB
• Volume: 49
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1994.1046

✦ Synopsis

A newly developed analytical technique to extract, derivatize, and simultaneously determine trace prostaglandins (PGs) in practical biological samples is described. Trace PGs were extracted by a column separation technique and derivatized by 9 -anthryldiazomethane (ADAM). The conventional chemical procedure for determination of carboxylic acids using ADAM was reinvestigated to get separation of trace (\mathrm{PGs}) from enormous amounts of coexisting biological substances and by- and decomposition products of ADAM. The PG derivatives were separated by high-performance liquid chromatography and detected by laser-induced fluorometry (HPLC/LIF). The minimum detectable absolute quantity of the HPLC/LIF system was (0.5 \mathrm{pg}(1.3 \mathrm{fmol})) per injection. For the overall chemical procedure, sub-nanogram per milliliter levels of PGs in human urine were determined simultaneously. O 1994 Academic Press, Inc.