Reverse-phase. high-performance liquid chromatography was used for the simultaneous determination of retinol, a-tocopherol, lycopene, a-carotene, and &carotene in human plasma. A multiple solvent system of methanol followed by a mixture of metbanol:acetonitrile:chloroform (47:42: 11) provided clear
Simultaneous determination of retinol and tocopherols by high-performance liquid chromatography
โ Scribed by Yukiko Satomura; Mieko Kimura; Yoshinori Itokawa
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 357 KB
- Volume
- 625
- Category
- Article
- ISSN
- 1873-3778
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๐ SIMILAR VOLUMES
An isocratic, high-performance liquid chromatography method is presented for the simultaneous analysis of retinol, tocopherols, carotenes, and other carotenoids. The method employs detection at two wavelengths (292 and 450 nm) and three internal standards, which are: retinyl acetate, alpha-tocophery
In this report we describe a modified reverse phase HPLC method that avoids the solvent evaporation step and allows simple and rapid determination of retinol, alpha-tocopherol, alpha-carotene and beta-carotene and achieves complete separation of alpha- and beta-carotene. Retinyl acetate, alpha-tocop