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Simultaneous determination of prednisolone, prednisolone acetate and hydrocortisone in human serum by high-performance liquid chromatography

✍ Scribed by S.A. Döppenschmitt; B. Scheidel; F. Harrison; J.P. Surmann


Publisher
Elsevier Science
Year
1995
Weight
551 KB
Volume
674
Category
Article
ISSN
0378-4347

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A method is described for the measurement of prednisone and prednisolone in human plasma by high-performance liquid chromatography (hplc). An ether/dichloromethane extract (60:40, v/v) of plasma is evaporated to dryness and chromatographed on a 250 x 4.5mm Whatman Partisil column with uv detection a