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Simultaneous determination of ondansetron and tropisetron in human plasma using HPLC with UV detection

✍ Scribed by Steffen Bauer; Elke Störmer; Rolf Kaiser; Pierre-Benoit Tremblay; Jürgen Brockmöller; Ivar Roots

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
76 KB
Volume
16
Category
Article
ISSN
0269-3879

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✦ Synopsis

Abstract

A rapid and sensitve HPLC method for the simultaneous quantitation of ondansetron and tropisetron, two serotonin (5‐HT) receptor antagonists frequently used in treatment and prevention of nausea and emesis, is described. The procedure involves liqid–liquid extraction of human plasma with dichloromethane coupled with reversed‐phase HPLC and UV detection. The lower limits of quantification (LOQ) were 0.62 ng/mL for ondansetron and 1.25 ng/mL or tropisetron. Intra‐ and inter‐assay coefficients of variation ranged from 1.5 to 7.5% and 5.3 to 13.7%, respectively. The sensitivity and precision were sufficient for determination of plasma concentrations after therapeutic administration of both drugs and the method can be used for the estimation of pharmacokinetic parameters. Copyright © 2002 John Wiley & Sons, Ltd.

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